Genome Editing with CRISPR-Cas9

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This cluster introduces learners to the powerful tool of gene editing called CRISPR-Cas9. In an application of this revolutionary technique, students generate immune cells that are resistant to HIV infection. A series of experiments teach the learner guide RNA design, sequence analysis of edited cells, determining protein expression by Western blot, and fluorescence microscopy.
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    Cambridge Science Festival, the first of its kind in the United States, is a madcap celebration of science, technology, engineering, art, and math (STEAM). A multifaceted, multicultural event, the Festival makes science accessible, interactive, and fun, highlighting the impact of STEAM in all our lives.

    For the 2021 Festival, we will host a month-long celebration of STEAM in our community with an overarching theme of gratitude and appreciation. During the month of April 2021, we will showcase creative digital and virtual entries from our rich STEAM community.

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